Analysis Note

Glutathione Quantification Kit contains a GSH masking reagent. This enables GSSG detection in the sample by measuring the absorption (λmax = 412 nm) obtained from a colorimetric reaction of DTNB (5,5'-dithiobis (2-nitrobenzoic acid)) coupled with the enzymatic recycling system. The total quantity of GSH can be measured by subtracting the amount of GSSG from the total amount of glutathione.

Application

Glutathione Quantification Kit is used to detect the total amount of glutathione as an index for oxidative stress or disease risk.

Components

Glutathione Quantification Kit contains: Enzyme Solution (50µl X 1) Buffer Solution (60ml X 1) Standard GSH (X 1) Masking Reagent (20µl X 1) Co-enzyme (X 2) Substrate (DTNB) (X4) Standard GSSG (X 1)

Features and Benefits

The detection ranges of total glutathione and GSSG using in this kit are from 0.5 µmol/l to 50 µmol/l and from 0.5 µmol/l to 25 µmol/l, respectively.

General description

Glutathione Quantification Kit is designed to detect oxidized and reduced Glutathione. Glutathione is usually represented in a reduced form called GSH; however, GSH is converted into its oxidized state (GSSG) upon oxidative stress. The ratio between GSH and GSSG is considered an index for oxidative stress. Glutathione, also known as γ-L-glutamyl-L-cysteinylglycine, is a tripeptide compound that is involved in anti-oxidation and drug metabolism. It acts as a substrate for enzymes like glutathione peroxidase, glutathione S-transferase, and thiol transferase.

Other Notes

not available in Korea, Singapore, Taiwan and Japan